{"id":37,"date":"2026-07-14T18:10:15","date_gmt":"2026-07-14T18:10:15","guid":{"rendered":"https:\/\/blitzlab.eu\/wissen-reinheit-hplc-ms\/"},"modified":"2026-10-23T23:36:59","modified_gmt":"2026-10-23T21:36:59","slug":"peptide-purity-hplc-ms","status":"publish","type":"page","link":"https:\/\/blitzlab.eu\/en\/wissen-reinheit-hplc-ms\/","title":{"rendered":"Reinheit verstehen: HPLC &#038; LC-MS"},"content":{"rendered":"<main class=\"container legal-wrap article\">\n  <div class=\"breadcrumb\"><a href=\"\/en\/\">Home<\/a><span class=\"sep\">\u203a<\/span><a href=\"\/en\/blog\/\">Blog<\/a><span class=\"sep\">\u203a<\/span><span>Purity: HPLC &amp; LC-MS<\/span><\/div>\n\n  <h1>Peptide Purity: HPLC and LC-MS Explained Simply<\/h1>\n  <p class=\"legal-lead\">Two analytical methods answer the key quality questions about a peptide batch: \"Is it the right molecule?\" and \"How pure is it?\" Here's what the values mean.<\/p>\n  <div class=\"legal-flag\"><svg class=\"bl-i\" width=\"16\" height=\"16\" viewbox=\"0 0 24 24\" fill=\"none\" stroke=\"currentColor\" stroke-width=\"1.8\" stroke-linecap=\"round\" stroke-linejoin=\"round\" aria-hidden=\"true\" focusable=\"false\"><circle cx=\"12\" cy=\"12\" r=\"9\"\/><path d=\"M12 11v5\"\/><path d=\"M12 7.6h.01\"\/><\/svg> General, scientific, factual information. No health, dosage, or application advice. Research Use Only.<\/div>\n\n  <h2>Why Purity Matters in Research<\/h2>\n  <p>In laboratory research, <strong>Reproducibility<\/strong> crucial. An impure or incorrectly identified substance can falsify results without it being immediately obvious. Therefore, each batch is analytically characterised before use \u2013 documented in <a href=\"\/en\/reading-a-coa\/\">Certificate of Analysis (COA)<\/a>.<\/p>\n\n  <h2>HPLC \u2013 the purity<\/h2>\n  <p><strong>HPLC<\/strong> stands for High-Performance Liquid Chromatography. Simplified: The sample is passed through a separation column in which the individual components move at different speeds. The result is a <em>Chromatogram<\/em> with signal peaks. The large main peak is the target molecule; small side peaks are impurities. The area ratio gives the <strong>Purity percentage<\/strong> \u2013 for example, \"\u2265 99%\".<\/p>\n\n  <h2>LC-MS \u2013 the identity<\/h2>\n  <p><strong>LC-MS<\/strong> couples liquid chromatography with a mass spectrometer. The mass spectrometer determines the <strong>Molecular weight<\/strong> very precise. If the measured mass matches the theoretically expected mass of the peptide, then the <em>Identity<\/em> is confirmed: it is indeed the declared substance and not something else.<\/p>\n\n  <h2>How to read \"\u2265 99% purity\"<\/h2>\n  <ul>\n    <li>The value refers to the <strong>tested batch<\/strong> \u2013 not a blanket.<\/li>\n    <li>\"\u2265 99%\" means that at least 99% of the detected components are the target molecule.<\/li>\n    <li>The statement is only meaningful in conjunction with the <strong>identity confirmation<\/strong> for LC-MS \u2013 a high purity value for the wrong substance is worthless.<\/li>\n  <\/ul>\n\n  <h2>What the values don't say<\/h2>\n  <p>Purity and identity are <strong>analytical quality characteristics<\/strong>. They make no statement regarding suitability for human or animal use, efficacy, or safety. All products are intended solely for scientific in-vitro laboratory research (Research Use Only).<\/p>\n\n  <div class=\"article-cta\">\n    <div>\n      <strong>Purity per batch documented<\/strong>\n      <p>Each batch is tested externally by HPLC and LC-MS \u2013 the value is stated in the COA on the product page.<\/p>\n    <\/div>\n    <a href=\"\/en\/shop\/\" class=\"btn btn-blue\">Shop now <span>\u2192<\/span><\/a>\n  <\/div>\n\n  <div class=\"wiss-related\">\n    <h3>More on the blog<\/h3>\n    <ul>\n      <li><a href=\"\/en\/reading-a-coa\/\">Understanding and reading a Certificate of Analysis (COA)<\/a><\/li>\n      <li><a href=\"\/en\/spotting-a-reliable-supplier\/\">Identifying a Reputable Peptide Supplier: 7-Point Checklist<\/a><\/li>\n      <li><a href=\"\/en\/storage-and-handling\/\">Storing and Handling Research Peptides<\/a><\/li>\n    <\/ul>\n  <\/div>\n<\/main>","protected":false},"excerpt":{"rendered":"<p>Start&rsaquo;Blog&rsaquo;Reinheit: HPLC &amp; LC-MS Peptid-Reinheit: HPLC und LC-MS einfach erkl\u00e4rt Zwei analytische Verfahren beantworten die wichtigsten Qualit\u00e4tsfragen einer Peptid-Charge: \u201eIst es das richtige Molek\u00fcl?\u201c und \u201eWie rein ist es?\u201c. Hier ist, was die Werte bedeuten. Allgemeine, wissenschaftlich-faktische Information. Keine Gesundheits-, Dosierungs- oder Anwendungsberatung. Research Use Only. Warum Reinheit in der Forschung z\u00e4hlt In der Laborforschung [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-37","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/blitzlab.eu\/en\/wp-json\/wp\/v2\/pages\/37","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blitzlab.eu\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/blitzlab.eu\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/blitzlab.eu\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blitzlab.eu\/en\/wp-json\/wp\/v2\/comments?post=37"}],"version-history":[{"count":2,"href":"https:\/\/blitzlab.eu\/en\/wp-json\/wp\/v2\/pages\/37\/revisions"}],"predecessor-version":[{"id":271,"href":"https:\/\/blitzlab.eu\/en\/wp-json\/wp\/v2\/pages\/37\/revisions\/271"}],"wp:attachment":[{"href":"https:\/\/blitzlab.eu\/en\/wp-json\/wp\/v2\/media?parent=37"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}